2004 Center Publications

Below are the Center publications. Use the options to the left to select the year and limit the type of publication you would like to view.

Peer Reviewed Journal Publications

Bhunia, A.K., Geng, T., Lathrop, A., Valadez, A., Morgan, M.T., Optical Immunosensors for Detection of Listeria monocytogenes and Salmonella Enteritidis from Food. Proc. of SPIE. 2004 v. 5271, p. 1-6.
Bhunia, A.K., Robinson, J.P., Ladisch, M.R., Composite Surface for Blocking Bacterial Adsorption on Protein Biochips. Biotechnology and Bioengineering. 2003. v. 81(5), p. 618-624.
Bright, N.G., Carroll, R.J., Applegate, B.M., A Model System for Pathogen Detection Using a Two-Component Bacteriophage/Bioluminescent Signal Amplification Assay. In Monitoring Food Safety, Agriculture, and Plant Health. Proceedings of SPIE. 2004. v. 5271, p. 13-19.
Chang, W.J., Akin, D., Sedlak, M., Ladisch, M.R., Bashir, R., Poly(dimethylsiloxane) (PDMS) and Silicon Hybrid Biochip for Bacterial Culture. Biomedical Microdevices. 2003. v. 5(4), p. 281-290.
Geng, T., Kim, K.P., Sherman, D., Gomez, R., Bashir, R., Ladisch, M., Bhunia, A.K., Expression of cellular antigens of Listeria monocytogenes that react with monoclonal antibodies C11E9 and EM-7G1 under acid-, salt- or temperature-induced stress environments. Journal of Applied Microbiology. 2003. v. 95. p. 762-772.
Hahm, B.K., Maldonado, Y., Schreiber, E., Bhunia, A.K. Nakatsu, C.H., Subtyping of Foodborne and Environmental Isolates of Escherichia coli by Multiplex-PCR, rep-PCR, PFGE, Ribotyping and AFLP. Journal of Microbiological Methods. 2003. v. 53, p.387-399.
Han, Y., R. H. Linton, and P. E. Nelson, (2004) Effects of recovery, plating, and inoculation methods on quantification of Escherichia coli O157:H7 and Listeria monocytogenes from strawberries. J. Food Protection. (In Press)
Han, Y., T. L. Selby, K. K. Schneider, R. H. Linton, and P. E. Nelson., (2004) Decontamination of strawberries using batch and continuous chlorine dioxide gas treatments. J. Food Protection. (In press)
Huang, T., Chang, W.J., Akin, D., Gomez, R., Bashir, R., Mosier, N., Ladisch, M.R., Microfiber Assisted Fabrication of Microfluidic Channels Using Poly(dimethylsiloxane). American Institute of Chemical Engineers (AIChE) Journal. 2003. v. 49(11), p. 2984-2987.
Huang, T., Geng, T., Sturgis, J., Li, H., Gomez, R., Bashir, R., Bhunia, A.K., Robinson, J.P., Ladisch, M.R., Lysozyme for Capture of Microorganisms on Protein Biochips. Enzyme and Microbial Technology. 2003. v.33, p. 958-966.
Huang, T., Sturgis, J., Gomez, R., Geng, T., Bashir, R., Bhunia, A.K. Robinson, J.P., Ladisch, M.R., Composite Surface For Blocking Bacterial Adsorption on Protein Biochips. Biotechnol. Bioeng. 2003. v.81(5), p. 618-624.
Iyer, M.S., Cousin, M.A., Immunological detection of Fusarium species in cornmeal. Journal of Food Protection. 2003. v. 65. p. 451-456.
Jaradat, J.W., Bhunia, A.K., Adhesion, Invasion, and translocation characteristics of Listeria monocytogenes serotypes in a Caco-2 cell and mouse model. Applied and Environmental Microbiology. 2003. v. 69(6). p. 3640-3645.
Jaradat, Z.W., Wampler, J.L., Bhunia, A.K., A Listeria adhesion protein-deficient Listeria monocytogenes strain shows reduced adhesion primarily to intestinal cell lines. Medical Microbiology and Immunology. 2003. v. 192. p. 85-91.
Lasrado, J.A., Santerre, C.R., Stahl, J.R., Noltemeyer, T. Deardorff, D.C., Measurement of polychlorinated biphenyls in fish tissue by gas chromatography with electron capture detection and enzyme-linked immunosorbent assay. Journal of Food Protection. 2004. v. 67(6) p. 1209-1213.
Lasrado, J.A., Santerre, C.R., Zajicek, J.L., Stahl, J.R., Tillitt, D.E., Deardorff, D.C., Determination of PCBs in fish tissue using enzyme-linked immunosorbent assay (ELISA). Journal of Food Science. 2003. v. 68(1) p. 133-136.
Lathrop, A., Jaradat, Z.W., Haley, T., Bhunia, A.K., Characterization and Application of a Listeria monocytogenes Reactive Monoclonal Antibody C11E9 in a Resonant Mirror Biosensor. Journal of Immunological Methods. 2003. v. 281, p. 119-128.
Lathrop, A.L., Jaradat, Z.W., Haley, T., Bhunia, A.K., Characterization and application of a Listeria monocytogenes reactive monoclonal antibody C11E9 in a resonant mirror biosensor. Journal of Immunological Methods. 2003. v. 281. p.119-128.
Menon, A., Shroyer, M.L., Wampler, J.L., Chawan, C.B., Bhunia, A.K., In vitro Study of Listeria monocytogenes Infection to Murine Primary and Human Transformed B Cells. Comparative Immunology, Microbiology & Infectious Diseases. 2003. v. 26. p. 157-174.
Shim, S.M., Santerre, C.R., Burgess, J., Deardorff, D.C., Omega-3 fatty acids and total PCB in 26 dietary supplements. Journal of Food Science. 2003. v. 68(8) p. 2436-2440.
Shim, S.M., Santerre, C.R., Dorworth, L.E., Miller, B.K., Stahl, J.R., Deardorff, D.C., Prediction of PCB content in sportfish using semipermeable membrane devices (SPMDs). Journal of Environmental Science and Health. 2004. Part B, B39(2) p. 263-271.
Shroyer, M., Bhunia, A.K., Development of a rapid 1-h fluorescence-based cytotoxicity assay for Listeria species. Journal of Microbiological Methods. 2003. v. 55. p. 35-40.
Singh, N., Singh, R.K., Bhunia, A.K., Sequential disinfection of Escherichia coli O157:H7 inoculated alfalfa seeds before and during sprouting using aqueous chlorine dioxide, ozonated water and thyme essential oil. Lebensmittel-Wissenschaft & Technologie. 2003. v. 36. p. 235-243.
Wampler, J.L., Kim, K.P., Jaradat, Z.W., Bhunia, A.K., Heat-shock protein 60 acts as a receptor for the Listeria adhesion protein in Caco-2 cells. Infection and Immunity. 2004. v. 72(2). p. 931-936.
Zhang, P., Wampler, J.L., Bhunia, A.K., Burkholder, K.M., Patterson, J.A., Whistler, R.L., Effects of arabinoxylans on activation of murine macrophages and growth performance of broiler chicks. Cereal Chemistry. 2004. v. 81(4). p. 511-514.

Abstracts for major papers/posters presented

Applegate, B.M., Detection of pathogenic bacteria by exploiting bacteriophage specificity and the Vibrio fischeri quorum system. The 226th ACS National Meeting, New York, NY. 2003. Abstract 100.
Bashir, R., Akin, D., Gomez, R., Li, H., Chang, W.J., Gupta, A., From BioMEMS to Bionanotechnology: Integrated Biochips for the Detection of Cells and Microorganisms. Materials Research Society Symposium Proceedings, v. 773. p. 117-124 San Francisco, CA, 2003. (Invited Paper).
Bhunia, A.K., Study of Listeria monocytogenes survival / inactivation on a new conveyor design. Institute of Food Technologist General Meeting. 2003. Abstract p. 85.
Bhunia, A.K., Geng, T., Lathrop, A. Valadez, A., Morgan, M.T., Optical immunosensors for detection of Listeria monocytogenes and Salmonella Enteritidis from food. Proceeding of SPIE. 2004. v. 5271. p. 1-6.
Burgula, Y., Khali, D., Kim, S., Cousin, M.A., Reuhs, B.R., Mauer, L.J., Development of a method to detect Salmonella Typhimurium using FT-IR spectroscopy. International Food Safety and Quality Conference. 2003. Abstract.
Burgula, Y., Khali, D., Kim, S., Cousin, M.A., Reuhs, B.R., Mauer, L.J., Development of a method to improve the sensitivity and selectivity for detection of E. coli O157:H7 using FT-IR spectroscopy. Institute of Food Technologists’ Annual Meeting and Food Expo. 2004. Abstract 25534, Session 114D, poster no. 19.
Burgula, Y., Khali, D.M., Kim, S., Cousin, M.A., Reuhs, B.L., Mauer, L.J., A rapid method for detection of E. coli O157:H7 using Dynabeads® and FTIR spectroscopy. International Association for Food Protection (IAFP) 91st Annual Conference. 2004. Abstract. Poster no. 145.
Chen, W., Ladisch, M.R., Geng, T., Bhunia, A.K., Biofunctional Membranes for Listeria monocytogenes Detection, BIOT Division Paper 112, 225th ACS National Meeting, Section: Advances in Bioseparations, New Orleans. 2003.
Davis, K.C., Bhunia, A.K., Detection of pathogenic Escherichia coli in food and environmental samples using PCR. American Society for Microbiology General Meeting. 2003. Abstract p. 494-495.
Geng, T., Kim, K.P., Morgan, M.T., Bhunia. A.K., Antibody-based rapid detection of low levels of Listeria monocytogenes cells using a fiber optic biosensor. Institute of Food Technologist General Meeting. 2003. Abstract No. 76E-3.
Gray, K.N., O’Neal, E., Bhunia, A.K., Quantitative detection of Bacillus cereus enterotoxin in 15 minutes using a mammalian cell enzyme release assay. American Society for Microbiology General Meeting. 2004. Abstract p. 498.
Hahm, B.K., Geng, T., Bhunia. A.K., Effect of environmental stress of antibody-based detection of Escherichia coli O157:H7, Salmonella Enteritidis and Listeria monocytogenes cells. American Society for Microbiology General Meeting. 2004. Abstract p. 499.
Huang, T., Chang, W.J., Akin, D., Gomez, R., Bashir, R., Mosier, N., Ladisch, M.R., Fabrication of Microfluidic Channels Using Microfibers with Poly(dimethylsiloxane), Poster Session: Advances in Biosensors and Biomedical Sensors I, Paper 193a, AIChE Annual Meeting, San Francisco, CA. 2003.
Huang, T.T., Geng, T., Akin, D., Chang, W., Sturgis, J., Bashir, R., Bhunia, A.K., Robinson, J.P., Ladisch, M.R., Biomediated Assembly of Functionalized Microbeads for Capture of Microorganisms. BIOT Division Paper 97, 225th ACS National Meeting, Section: Novel Bioanalyses Using Lab-on-a-Chip Technologies, New Orleans. 2003.
Jaradat, Z.W., Bhunia, A.K., Variation in adhesion and invasion behavior among different serotypes of Listeria monocytogenes to Caco-2 cells. American Society for Microbiology General Meeting. 2003. Abstract p. 376.
Kim, K.P., Geng, T., Bhunia, A.K., Negative Regulation of LepA in Listeria monocytogenes Survival and Growth in Stress Conditions. Institute of Food Technologist General Meeting, Chicago, IL. 2003. Abstract 29G-13, p. 83.
Kim, K.P., Jaradat, J.W., Bhunia, A.K., Alcohol acetaldehyde dehydrogenase homologue of Listeria monocytogenes is a Listeria adhesion protein (LAP) involved in pathogenesis. American Society for Microbiology General Meeting. 2003. Abstract p. 490.
Kim, S., Burgula, Y., Ojaanen-Reuhs, T., Ruehs, B.R., Cousin, M.A., Mauer, L.J., Differentiation and classification of the phenol-phase soluble crude lipopolysaccharides from Eschericia coli strains using Fourier transform infrared spectroscopy and canonical variate analysis. Institute of Food Technologists’ Annual Meeting and Food Expo. 2004. Abstract.
Lathrop, A., Kim, K.P., Bhunia, A.K., Development of Listeria monocytogenes specific antibodies through the use of genomics/proteomics. American Society for Microbiology General Meeting. 2004. Abstract p. 494.
Maldonado, Y., Bhunia, A.K., Adhesion and cytotoxic properties of enterohemorrhagic E. coli. American Society for Microbiology General Meeting. 2003. Abstract p. 490.
Minocha, U., Bright, N., Perry, L., Applegate, B., Detection of the Foodborne Pathogen Escherchia coli O157:H7 Using an ainS Recombinant phiV10 Bacteriophage Based Bioluminescent Reporter System. ASM Biodefense Research meeting: Future Directions for Biodefense Research: Development of Countermeasures. Baltimore, MD. 2004. Abstract 100.
Minocha, U., Perry, L.L., Jennings, R., Bhunia, A., Applegate, B., Genome Sequence Analysis and Evaluation of Strain Specificity of E. coli O157:H7 Bacteriophage phi V10. American Society for Microbiology, New Orleans, LA. 2004. Abstract M028.
Naschansky, K.N., Budiarty, S., Sharma, A., Morgan, M., Bashir, R., Bhunia, A.K., Rapid detection of Listeria monocytogenes in naturally contaminated food samples through enzymatic and biosensor-based cytotoxicity analysis. American Society for Microbiology General Meeting. 2003. Abstract p. 497.
Padmapriya, B.P., Geng, T. Chen, W. Liu, L. Ladisch, M. R. Bhunia, A.K., Concentration of Listeria monocytogenes cells directly from meat samples using cell concentration and recovery (CCR) kit and detection by PCR and fiber optic biosensor. International Conference on Food Safety. 2003. Paper No.
Perry, L.L., Bright, N.G., P. del Busto, M.D., Applegate, B.M., Comparison of the LuxR Proteins and Associated Operators from Vibrio fischeri Strains MJ-1 and ATCC 49387 (Microtox assay strain). American Society for Microbiology, New Orleans, LA. 2004. Abstract K056.
Shroyer, M., Minocha, U., Lino, F., Bright, N., Perry, L., Applegate, B., Development of a Recombination System for Rapid Construction of E. coli O157:H7 Reporter Bacteriophage. American Society for Microbiology, New Orleans, LA. 2004. Abstract M017.
Wampler, J.L., Kim, K.P., Bhunia, A.K., Identification of Hsp60 as a putative receptor for the Listeria adhesion protein in Caco-2 cells. American Society for Microbiology General Meeting. 2003. Abstract p. 490.

Thesis/Dissertations

Gaba, Anjali, Agricultural and Biological Engineering. Bioprocess Engineering. M.S., (2000-2003), Covalent Immobilization of Antibody C11E9 on a Silica Surface for Use in a Biosensor and Capture of Antigen Listeria Monocytogenes Using the Immobilized Antibody.
Geng, T., Study of antibodies to stress induced cellular antigens of Listeria monocytogenes and its detection using a fiber-optic biosensor in food. PhD Dissertation. 2004. Purdue University. 140 p.
Geng, Tao, Food Science, Ph.D., (2001-2004), Study of Antibodies to Stress Induced Cellular Antigens of Listeria monocytogenes and its Detection Using a Fiber-Optic Biosensor in Food. Dissertation Defended: April 6, 2004.
Gomez, Rafael, Electrical and Computer Engineering, Ph.D., (2001-2003), Microfabricated Device for Impedance-Based Electronic Detection of Bacterial Metabolism. Dissertation Defended: November 26, 2003.
Guo, S., Optical scattering for bacterial colony detection and characterization. Master of Science Dissertation. 2004. Purdue University. 98 p.
Maldonado, Y., Characterization of Escherichia coli from food, clinical and environmental sources by genomic typing, cytotoxicity analysis and by adhesion to eukaryotic cells. MS Thesis. 2004. 116 p.
Naschansky, K.M., Cytotoxicity and cell-based sensors for detection of Listeria monocytogenes and Bacillus cereus. PhD Dissertation. 2004. Purdue University. 115 p.
Vierra, J. M., Detection of Fusarium graminearum and Fusarium verticillioides by immunocapture real-time PCR. M.S. Thesis. 2004. Purdue University. 69 p.
Wampler, J.L., Listeria monocytogenes adhesion to intestinal cells is mediated in part, by interaction of Listeria adhesion protein with a eukaryotic cell receptor, heat shock protein 60. PhD Dissertation. 2003. Purdue University. 86 p.

Books and Book Chapters

Bhunia, A.K., Lathrop. A., Foodborne Pathogen Detection, McGraw-Hill Professional, New York, NY. McGraw-Hill 2003 Year Book of Science and Technology, 2003. p.320-323.

Patents Granted

Bashir, R., Gomez,R., Robinson, P., Bhunia, A., Ladisch, M.R., Biosensor and Related Method, US Patent 6,716,620 B2 (April 6, 2004).
Sayler, Gary S., Ripp, Steven A., Applegate, B., Bioluminescent biosensor device. U.S. Patent 6,544,729.